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superblock blocking buffers  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superblock blocking buffers
    Superblock Blocking Buffers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+buffer/BSA+Blocking+Buffer%2C+3%25+in+TBS/pmc13019076-408-27-30
    Average 96 stars, based on 1 article reviews
    superblock blocking buffers - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Isolation and Characterization of Integrin α9 Positive Extracellular Vesicles Derived from Human Corneoscleral Rings
    Article Snippet: Samples were separated by 10% SDS-polyacrylamide gel electrophoresis and transferred onto PVDF transfer membrane (PerkinElmer, NEF1002001PK, Waltham, MA, USA). .. After blocking for 1 h in 5% BSA buffer, primary antibodies (CD9, Cell Signalling, #13403, 1:1000, Danvers, MA, USA; CD63, Novus, MX-49.129.5, 1:1000, Centennial, CO, USA; CD81, Cell Signalling, #52892, 1:1000; Alix, Cell Signalling, #92880, 1:1000; ABCG2, Thermo, 13-8888-82, 1:500; P63-α, Cell Signalling, #13109, 1:1000; Notch-1, Invitrogen, # MA5-32080, 1:1000, Waltham, MA, USA; Integrin alpha 9, Abcam, ab140599, 1:1000, Cambridge, UK) was added for overnight incubation at 4 °C. .. HRP-secondary antibody was added for 1 h at room temperature, and ECL substrate (A38556, Thermo Fisher Scientific) was added for chemiluminescence.

    Article Title: Chromogranin A and catestatin regulate pancreatic islet homeostasis, endocrine function, and neurotransmitter signaling
    Article Snippet: Afterwards, for each condition 60 μg of protein was loaded and run on 10% mini-protein TGX gels (Bio-Rad; 4561033) in Tris/Tricine/SDS buffer (Bio-Rad; 1610744) followed by transfer for 60 min at 100 V at 4 °C in Tris/Glycine buffer (Bio-Rad; 1610771) with methanol (Supelco; 1263283323) using a PVDF membrane. .. The membranes were taken out the cassette and washed with distilled water, followed by blocking in 3% BSA buffer for 1 h at r.t. Membranes were stained o/n at 4°C with primary antibody rabbit anti-CgA (1:1000, Invitrogen; PA5-35071) or rabbit anti-CST (1:1000, Proteintech; 289-MM-0288). ..

    Article Title: Targeted Delivery of c(RGDfk)‐Modified Liposomes to Bone Marrow Through In Vivo Hitchhiking Neutrophils for Multiple Myeloma Therapy
    Article Snippet: .. Following blocking with 3% BSA buffer, the slices were incubated overnight with primary antibodies, including anti‐IL‐6 antibody (Invitrogen, P620), anti‐IL‐1 β antibody (Invitrogen, P420B), and anti‐TNF‐ α antibodies (Invitrogen, PA5‐19810), respectively. ..

    Article Title: Schistosoma japonicum cathepsin L1: A potential target for anti-schistosomiasis strategies
    Article Snippet: Total IgG from sera of rabbits immunized with rSjCL1 was purified using the rProtein A Sepharose Fast Flow Kit (GE Healthcare, Uppsala, Sweden) according to the manufacturer’s instructions. .. After blocking with 5% BSA buffer (in PBS) for 2 h at 25°C, the sections were incubated with rabbit polyclonal antibodies against rSjCL1 (2 mg/mL, 1:500 dilutions) and IgG from a non-immunized rabbit in blocking solution overnight at 4°C, followed by incubation with Alexa Fluor 555 donkey anti-rabbit IgG (H + L) (1:1,000 dilution, Invitrogen) for 1 h at 25°C. .. The sections were finally stained with 50 μL Fluoroshield Mounting Medium with DAPI (Abcam) and visualized immediately with a TCS SP5 confocal microscope (Leica Microsystems, Wetzlar, Germany).

    Article Title: Schistosoma japonicum cathepsin L1: A potential target for anti-schistosomiasis strategies.
    Article Snippet: Total IgG from sera of rabbits immunized with rSjCL1 was purified using the rProtein A Sepharose Fast Flow Kit (GE Healthcare, Uppsala, Sweden) according to the manufacturer’s instructions. .. After blocking with 5% BSA buffer (in PBS) for 2 h at 25°C, the sections were incubated with rabbit polyclonal antibodies against rSjCL1 (2 mg/mL, 1:500 dilutions) and IgG from a non-immunized rabbit in blocking solution overnight at 4°C, followed by incubation with Alexa Fluor 555 donkey anti-rabbit IgG (H + L) (1:1,000 dilution, Invitrogen) for 1 h at 25°C. .. The sections were finally stained with 50 μL Fluoroshield Mounting Medium with DAPI (Abcam) and visualized immediately with a TCS SP5 confocal microscope (Leica Microsystems, Wetzlar, Germany).

    Incubation:

    Article Title: Isolation and Characterization of Integrin α9 Positive Extracellular Vesicles Derived from Human Corneoscleral Rings
    Article Snippet: Samples were separated by 10% SDS-polyacrylamide gel electrophoresis and transferred onto PVDF transfer membrane (PerkinElmer, NEF1002001PK, Waltham, MA, USA). .. After blocking for 1 h in 5% BSA buffer, primary antibodies (CD9, Cell Signalling, #13403, 1:1000, Danvers, MA, USA; CD63, Novus, MX-49.129.5, 1:1000, Centennial, CO, USA; CD81, Cell Signalling, #52892, 1:1000; Alix, Cell Signalling, #92880, 1:1000; ABCG2, Thermo, 13-8888-82, 1:500; P63-α, Cell Signalling, #13109, 1:1000; Notch-1, Invitrogen, # MA5-32080, 1:1000, Waltham, MA, USA; Integrin alpha 9, Abcam, ab140599, 1:1000, Cambridge, UK) was added for overnight incubation at 4 °C. .. HRP-secondary antibody was added for 1 h at room temperature, and ECL substrate (A38556, Thermo Fisher Scientific) was added for chemiluminescence.

    Article Title: Targeted Delivery of c(RGDfk)‐Modified Liposomes to Bone Marrow Through In Vivo Hitchhiking Neutrophils for Multiple Myeloma Therapy
    Article Snippet: .. Following blocking with 3% BSA buffer, the slices were incubated overnight with primary antibodies, including anti‐IL‐6 antibody (Invitrogen, P620), anti‐IL‐1 β antibody (Invitrogen, P420B), and anti‐TNF‐ α antibodies (Invitrogen, PA5‐19810), respectively. ..

    Article Title: Schistosoma japonicum cathepsin L1: A potential target for anti-schistosomiasis strategies
    Article Snippet: Total IgG from sera of rabbits immunized with rSjCL1 was purified using the rProtein A Sepharose Fast Flow Kit (GE Healthcare, Uppsala, Sweden) according to the manufacturer’s instructions. .. After blocking with 5% BSA buffer (in PBS) for 2 h at 25°C, the sections were incubated with rabbit polyclonal antibodies against rSjCL1 (2 mg/mL, 1:500 dilutions) and IgG from a non-immunized rabbit in blocking solution overnight at 4°C, followed by incubation with Alexa Fluor 555 donkey anti-rabbit IgG (H + L) (1:1,000 dilution, Invitrogen) for 1 h at 25°C. .. The sections were finally stained with 50 μL Fluoroshield Mounting Medium with DAPI (Abcam) and visualized immediately with a TCS SP5 confocal microscope (Leica Microsystems, Wetzlar, Germany).

    Article Title: Schistosoma japonicum cathepsin L1: A potential target for anti-schistosomiasis strategies.
    Article Snippet: Total IgG from sera of rabbits immunized with rSjCL1 was purified using the rProtein A Sepharose Fast Flow Kit (GE Healthcare, Uppsala, Sweden) according to the manufacturer’s instructions. .. After blocking with 5% BSA buffer (in PBS) for 2 h at 25°C, the sections were incubated with rabbit polyclonal antibodies against rSjCL1 (2 mg/mL, 1:500 dilutions) and IgG from a non-immunized rabbit in blocking solution overnight at 4°C, followed by incubation with Alexa Fluor 555 donkey anti-rabbit IgG (H + L) (1:1,000 dilution, Invitrogen) for 1 h at 25°C. .. The sections were finally stained with 50 μL Fluoroshield Mounting Medium with DAPI (Abcam) and visualized immediately with a TCS SP5 confocal microscope (Leica Microsystems, Wetzlar, Germany).

    Staining:

    Article Title: Chromogranin A and catestatin regulate pancreatic islet homeostasis, endocrine function, and neurotransmitter signaling
    Article Snippet: Afterwards, for each condition 60 μg of protein was loaded and run on 10% mini-protein TGX gels (Bio-Rad; 4561033) in Tris/Tricine/SDS buffer (Bio-Rad; 1610744) followed by transfer for 60 min at 100 V at 4 °C in Tris/Glycine buffer (Bio-Rad; 1610771) with methanol (Supelco; 1263283323) using a PVDF membrane. .. The membranes were taken out the cassette and washed with distilled water, followed by blocking in 3% BSA buffer for 1 h at r.t. Membranes were stained o/n at 4°C with primary antibody rabbit anti-CgA (1:1000, Invitrogen; PA5-35071) or rabbit anti-CST (1:1000, Proteintech; 289-MM-0288). ..

    Article Title: Somatic mutations in IRE1α regulate keratinocyte migration and survival by differentially activating Rho GTPases
    Article Snippet: .. The cells were then permeabilized in 1% BSA buffer containing 0.3% Triton X-100 for 10 min, blocked with 1% BSA and stained with 1× Alexa Fluor TM 488 Phalloidin (Invitrogen) for 45 min at RT. .. The cells were mounted using with DAPI mounting medium (VectorLabs) and were visualized with a LSM880 Fluorescence microscope with Airyscan (Zeiss) using the 63× oil-immersion objective.

    Single Cell:

    Article Title: Sorting nexin 5 mediates antigen presentation and immunity against Mycobacterium tuberculosis
    Article Snippet: Lungs from WT and Snx5 −/− mice uninfected or infected with Mtb were minced and incubated in PBS with 0.5% BSA, 0.2 mg/mL DNase I (Sigma, DN25), and 5mg/mL collagenase type IV (Gibco, 17102-015) on an orbital shaker at 100 rpm at 37 °C for 30 min. After digestion, the lung tissues were processed through a 70 μm cell strainer on a 50 mL conical tube. .. Then, the filters were washed with BSA buffer (PBS + 0.5% BSA), and the single-cell suspension was centrifuged at 350 × g for 8 min at 4 °C, and then resuspended in 1 mL of ACK lysis buffer (Gibco, A10492-01). ..

    Suspension:

    Article Title: Sorting nexin 5 mediates antigen presentation and immunity against Mycobacterium tuberculosis
    Article Snippet: Lungs from WT and Snx5 −/− mice uninfected or infected with Mtb were minced and incubated in PBS with 0.5% BSA, 0.2 mg/mL DNase I (Sigma, DN25), and 5mg/mL collagenase type IV (Gibco, 17102-015) on an orbital shaker at 100 rpm at 37 °C for 30 min. After digestion, the lung tissues were processed through a 70 μm cell strainer on a 50 mL conical tube. .. Then, the filters were washed with BSA buffer (PBS + 0.5% BSA), and the single-cell suspension was centrifuged at 350 × g for 8 min at 4 °C, and then resuspended in 1 mL of ACK lysis buffer (Gibco, A10492-01). ..

    Lysis:

    Article Title: Sorting nexin 5 mediates antigen presentation and immunity against Mycobacterium tuberculosis
    Article Snippet: Lungs from WT and Snx5 −/− mice uninfected or infected with Mtb were minced and incubated in PBS with 0.5% BSA, 0.2 mg/mL DNase I (Sigma, DN25), and 5mg/mL collagenase type IV (Gibco, 17102-015) on an orbital shaker at 100 rpm at 37 °C for 30 min. After digestion, the lung tissues were processed through a 70 μm cell strainer on a 50 mL conical tube. .. Then, the filters were washed with BSA buffer (PBS + 0.5% BSA), and the single-cell suspension was centrifuged at 350 × g for 8 min at 4 °C, and then resuspended in 1 mL of ACK lysis buffer (Gibco, A10492-01). ..



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